Highly efficient destruction of squamous carcinoma cells of the head and neck by photochemical internalization of Ranpirnase.
J Exp Ther Oncol
; 12(2): 113-120, 2017 Nov.
Article
en En
| MEDLINE
| ID: mdl-29161778
ABSTRACT
INTRODUCTION:
Photochemical Internalization is a novel drug delivery technology for cancer treatment based on the principle of Photodynamic Treatment. Using a photosensitizer that locates in endocytic vesicles membranes of tumor cells, Photochemical internalization enables cytosolic release of endocytosed antitumor agents in a site-specific manner. The purpose of the present in-vitro study was to explore whether Photochemical Internalization is able to enhance the efficacy of Ranpirnase, a cytotoxic amphibian ribonuclease, for eradication of squamous cell carcinoma of the head and neck.METHODS:
Cell viability was measured in 8 primary human cell lines of squamous cell carcinoma of the head and neck after treatment with Ranpirnase and Photochemical Internalization. For Photochemical Internalization the photosensitizer disulfonated tetraphenyl porphine was incubated with tumor cells followed by exposure to blue light (435 nm).RESULTS:
Our study demonstrates significant enhancement of antitumor activity of Ranpirnase by Photochemical Internalization. Treatment responses were heterogeneous between the primary cancer cell lines. Combining Photochemical Internalization with Ranpirnase resulted in 4.6 to 1,940-fold increased cytotoxicity when compared with the ribonuclease alone (P < 0.05).CONCLUSION:
Cytotoxicity of Ranpirnase can be markedly enhanced by Photochemical Internalization in squamous cell carcinoma of the head and neck.Palabras clave
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Banco de datos:
MEDLINE
Asunto principal:
Fotoquimioterapia
/
Ribonucleasas
/
Carcinoma de Células Escamosas
/
Neoplasias de Cabeza y Cuello
/
Antineoplásicos
Límite:
Humans
Idioma:
En
Revista:
J Exp Ther Oncol
Asunto de la revista:
NEOPLASIAS
/
TERAPIA POR MEDICAMENTOS
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania