Quantification of glycerophospholipids and sphingomyelin in human milk and infant formula by high performance liquid chromatography coupled with mass spectrometer detector.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1072: 235-243, 2018 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-29195142
ABSTRACT
Phospholipids and sphingomyelin have a central role in infant nutrition, phospholipid acting as a nutrient carrier of long chain polyunsaturated fatty acids and sphingomyelin having an important role in cognitive function. However, analytical methods to precisely characterize and quantify these compounds in maternal milk are needed. Phospholipids and sphingomyelin were extracted using chloroform and methanol and separated on Polaris 3 Si column 250×2.0mm from Varian and analyzed by high performance liquid chromatography (HPLC) coupled with mass spectrometer detector (MS). The analytical method was validated and repeatability, intermediate reproducibility, and recovery values were calculated. The relative standard deviation of repeatability (CV(r)) and intermediate reproducibility (CV(iR)) values ranged between 2.3 and 7.2% and 9.5 and 17.8%, respectively and the recovery values between 96 and 109%. Finally, the validated method was tested on human milk samples and on infant formula which were analysed also by HPLC coupled with evaporative light scattering detector (ELSD). In human milk, sphingomyelin (9.28mg100mL-1) was the most abundant compound, followed by phosphatidylcholine (5.39mg100mL-1), phosphatidylethanolamine (2.85mg100mL-1) and phosphatidylinositol (1.82mg100mL-1).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Esfingomielinas
/
Cromatografía Líquida de Alta Presión
/
Glicerofosfolípidos
/
Fórmulas Infantiles
/
Leche Humana
Tipo de estudio:
Prognostic_studies
Límite:
Humans
/
Infant
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2018
Tipo del documento:
Article