CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.

Chen, Janice S; Ma, Enbo; Harrington, Lucas B; Da Costa, Maria; Tian, Xinran; Palefsky, Joel M; Doudna, Jennifer A

Chen, Janice S; Ma, Enbo; Harrington, Lucas B; Da Costa, Maria; Tian, Xinran; Palefsky, Joel M; Doudna, Jennifer A.

Afiliación

Chen JS; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Ma E; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Harrington LB; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Da Costa M; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Tian X; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Palefsky JM; Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Doudna JA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. doudna@berkeley.edu.

Science ; 360(6387): 436-439, 2018 04 27.

Article en En | MEDLINE | ID: mdl-29449511

Asunto(s)

Proteínas Bacterianas/química; Proteínas Asociadas a CRISPR/química; Sistemas CRISPR-Cas; Clostridiales/enzimología; División del ADN; ADN de Cadena Simple/química; Endonucleasas/química; Cinética; Especificidad por Sustrato

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / ADN de Cadena Simple / Endonucleasas / División del ADN / Proteínas Asociadas a CRISPR / Sistemas CRISPR-Cas / Clostridiales Idioma: En Revista: Science Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

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