Biosensing estrogenic endocrine disruptors in human blood and urine: A RAPID cell-free protein synthesis approach.
Toxicol Appl Pharmacol
; 345: 19-25, 2018 04 15.
Article
en En
| MEDLINE
| ID: mdl-29499249
ABSTRACT
Many diseases and disorders are linked to exposure to endocrine disrupting chemicals (EDCs) that mimic the function of natural estrogen hormones. Here we present a Rapid Adaptable Portable In-vitro Detection biosensor platform (RAPID) for detecting chemicals that interact with the human estrogen receptor ß (hERß). This biosensor consists of an allosteric fusion protein, which is expressed using cell-free protein synthesis technology and is directly assayed by a colorimetric response. The resultant biosensor successfully detected known EDCs of hERß (BPA, E2, and DPN) at similar or better detection range than an analogous cell-based biosensor, but in a fraction of time. We also engineered cell-free protein synthesis reactions with RNAse inhibitors to increase production yields in the presence of human blood and urine. The RAPID biosensor successfully detects EDCs in these human samples in the presence of RNAse inhibitors. Engineered cell-free protein synthesis facilitates the use of protein biosensors in complex sample matrices without cumbersome protein purification.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
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Técnicas Biosensibles
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Sistema Libre de Células
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Disruptores Endocrinos
Límite:
Humans
Idioma:
En
Revista:
Toxicol Appl Pharmacol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos