In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests.
Comb Chem High Throughput Screen
; 21(4): 262-270, 2018.
Article
en En
| MEDLINE
| ID: mdl-29600754
ABSTRACT
BACKGROUND:
More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.OBJECTIVE:
This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China. MATERIALS ANDMETHODS:
DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549.CONCLUSION:
The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Curtiembre
/
Sustancias Peligrosas
/
Pruebas de Micronúcleos
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Comb Chem High Throughput Screen
Asunto de la revista:
BIOLOGIA MOLECULAR
/
QUIMICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
China