Expression of vesicular glutamate transporters in transient receptor potential ankyrin 1 (TRPA1)-positive neurons in the rat trigeminal ganglion.
Brain Res
; 1690: 31-39, 2018 07 01.
Article
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| MEDLINE
| ID: mdl-29649466
ABSTRACT
Transient receptor potential ankyrin 1 (TRPA1), a cold receptor in sensory neurons activated by a variety of stimuli, is implicated in nociception and mechanotransduction. To help understand the vesicular glutamate transporter (VGLUT)-mediated glutamate signaling in TRPA1-immunopositive (+) neurons, we examined the expression of VGLUT1 and VGLUT2 in the TRPA1+ neurons in the male rat trigeminal ganglion (nâ¯=â¯19) under normal conditions and following experimental inflammation in the vibrissal pad by light microscopic immunohistochemistry (nâ¯=â¯11), western blot (nâ¯=â¯8), and quantitative analysis. One half (50.8%, 250/492) of the TRPA1+ neurons expressed VGLUT2, and a small fraction (8.3%, 57/683) also expressed VGLUT1. The majority of the VGLUT2-expressing TRPA1+ (VGLUT2+/TRPA1+) neurons coexpressed the markers of peptidergic and non-peptidergic neurons, CGRP, IB4, and TRPV1 but not the markers of neurons with myelinated fibers, NF200 and parvalbumin. In contrast, most VGLUT1+/TRPA1+ neurons coexpressed NF200 and parvalbumin but rarely expressed CGRP, IB4, or TRPV1. Following experimental inflammation, the fraction of VGLUT2+ (experimental vs. control 34.7% vs. 22.3%), TRPA1+ (39.3% vs. 25.3%), and VGLUT2+/TRPA1+ (60.7% vs. 49.7%) neurons and the protein levels for TRPA1 and VGLUT2 increased significantly, compared to control, whereas the fraction of VGLUT1+ and VGLUT1+/TRPA1+ neurons and the protein level for VGLUT1 remained unchanged. These findings suggest that both VGLUT1 and VGLUT2 are involved in the glutamate signaling in TRPA1+ neurons under normal conditions in the male rats, and raise a possibility that VGLUT2 may play a role in the TRPA1-induced hypersensitivity following inflammation.
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Banco de datos:
MEDLINE
Asunto principal:
Ganglio del Trigémino
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Proteína 1 de Transporte Vesicular de Glutamato
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Proteína 2 de Transporte Vesicular de Glutamato
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Canal Catiónico TRPA1
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Neuronas
Límite:
Animals
Idioma:
En
Revista:
Brain Res
Año:
2018
Tipo del documento:
Article