Imperatorin ameliorates lipopolysaccharide induced memory deficit by mitigating proinflammatory cytokines, oxidative stress and modulating brain-derived neurotropic factor.
Cytokine
; 110: 78-86, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-29705395
ABSTRACT
BACKGROUND:
Lipopolysaccharide (LPS), an endotoxin from the outer membrane of Gram negative bacteria has been reported to cause neuroinflammation and learning and memory deficits. There are reports describing the beneficial effects of Imperatorin (IMP), a naturally occurring furanocoumarin in central nervous system (CNS) disorders such as anxiety and epilepsy.OBJECTIVE:
In the current study, we investigated whether IMP protects against LPS mediated memory deficits and neuroinflammation.METHODS:
Mice pretreated with IMP (5, 10â¯mg/kg po) were administered LPS (250⯵g/kg ip) for 7â¯days. Memory was evaluated in the Morris water maze (MWM) and Y maze. The mice were euthanised and different biochemical assessments were carried out to measure oxidative stress and acetyl choline esterase (AChE). Further, evaluation of proinflammatory cytokines such as tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) levels and brain derived neurotrophic factor (BDNF) in hippocampus and cortex of brain were performed.RESULTS:
LPS administration caused poor memory retention in both, MWM and Y maze, and caused distinct oxidative stress since decrease in superoxide dismutase (SOD), reduced glutathione (GSH) levels and increased lipid peroxidation were observed. Also, a significant rise was observed in the levels of AChE. Moreover, a rise in TNF-α and IL-6 levels and depleted levels of BDNF were noted. IMP pretreatment reversed LPS induced behavioral and memory disturbances and significantly decreased the oxidative stress and AChE levels. It also reduced TNF-α and IL-6 levels and caused a significant upregulation of BDNF levels.CONCLUSION:
Present study highlights the potential neuroprotective role of IMP against LPS mediated cognitive impairment and neuroinflammation.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Furocumarinas
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Lipopolisacáridos
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Citocinas
/
Estrés Oxidativo
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Factor Neurotrófico Derivado del Encéfalo
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Memoria
/
Trastornos de la Memoria
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Cytokine
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
India