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Quantifying cell free DNA in urine: comparison between commercial kits, impact of gender and inter-individual variation.
Streleckiene, Greta; Reid, Hayley M; Arnold, Norbert; Bauerschlag, Dirk; Forster, Michael.
Afiliación
  • Streleckiene G; Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu Str. 2, LT-50009 Kaunas, Lithuania.
  • Reid HM; Institute of Clinical Molecular Biology, Christian-Albrechts-Universität zu Kiel, Schleswig-Holstein, D-24105 Kiel, Germany.
  • Arnold N; Institute of Clinical Molecular Biology, Christian-Albrechts-Universität zu Kiel, Schleswig-Holstein, D-24105 Kiel, Germany.
  • Bauerschlag D; Institute of Clinical Molecular Biology, Christian-Albrechts-Universität zu Kiel, Schleswig-Holstein, D-24105 Kiel, Germany.
  • Forster M; Department of Gynecology & Obstetrics, University Hospital of Schleswig-Holstein, Christian-Albrechts-Universität zu Kiel, D-24105 Kiel, Germany.
Biotechniques ; 64(5): 225-230, 2018 05.
Article en En | MEDLINE | ID: mdl-29793362
DNA can enter the blood circulation from living cells by extracellular vesicles or at cell death, and pass into urine through the kidney barrier. Urine can be collected non-invasively, making it an interesting source of cell-free DNA (cfDNA) for research studies and ultimately for clinical diagnostics. However, there is currently a lack of data on the quantity and variability of cfDNA in urine. Here, we benchmark two commercial urine cfDNA isolation kits with respect to the quantity of DNA, the labor time, and cost. The results show distinctive differences between each kit. Furthermore, the cfDNA amount from the same probands varied strongly from day to day and may be higher in female samples than in male samples (p = 0.003).
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Urinálisis / Ácidos Nucleicos Libres de Células Límite: Female / Humans / Male Idioma: En Revista: Biotechniques Año: 2018 Tipo del documento: Article País de afiliación: Lituania

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Urinálisis / Ácidos Nucleicos Libres de Células Límite: Female / Humans / Male Idioma: En Revista: Biotechniques Año: 2018 Tipo del documento: Article País de afiliación: Lituania