Determination of glyphosate residues in Egyptian soil samples.
Anal Biochem
; 557: 1-6, 2018 09 15.
Article
en En
| MEDLINE
| ID: mdl-30003881
ABSTRACT
A sensitive linker-assisted enzyme-linked immunosorbent assay (L'ELISA) was developed for the analysis of glyphosate in Egyptian soil samples. Polyclonal glyphosate antibodies were produced from rabbits immunized with glyphosate protein conjugate. The conjugate was prepared by activating the carboxylic groups of proteins; thyroglobulin or bovine serum albumin with 1-ethyl-3- (3-diaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide followed by directly coupled to the amino group of glyphosate. The L'ELISA used succinic anhydride to derivatize glyphosate, which mimics the epitopic attachment of glyphosate to thyroglobulin. L'ELISA recognized the derivatized glyphosate with a limit of detection (LOD) of 0.8â¯ngâ¯g-1 and sensitivity (IC50 value) of 0.018⯵gâ¯g-1. The recovery values of the spiked soil samples with different concentrations of glyphosate were in the range of 87.4-97.2%. Good correlation was achieved between L'ELISA and conventional high-pressure liquid chromatography (HPLC) with fluorescence detection. This study demonstrated the utility and convenience of the sensitive, simple, practical and cost-effective L'ELISA method for glyphosate analysis in soil samples. Also, it is ideal for rapid screening of a large number of environmental samples.
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Banco de datos:
MEDLINE
Asunto principal:
Suelo
/
Contaminantes del Suelo
/
Glicina
País/Región como asunto:
Africa
Idioma:
En
Revista:
Anal Biochem
Año:
2018
Tipo del documento:
Article