Influenza A Virus Negative Strand RNA Is Translated for CD8+ T Cell Immunosurveillance.
J Immunol
; 201(4): 1222-1228, 2018 08 15.
Article
en En
| MEDLINE
| ID: mdl-30012850
ABSTRACT
Probing the limits of CD8+ T cell immunosurveillance, we inserted the SIINFEKL peptide into influenza A virus (IAV)-negative strand gene segments. Although IAV genomic RNA is considered noncoding, there is a conserved, relatively long open reading frame present in segment 8, encoding a potential protein termed NEG8. The biosynthesis of NEG8 from IAV has yet to be demonstrated. Although we failed to detect NEG8 protein expression in IAV-infected mouse cells, cell surface Kb-SIINFEKL complexes are generated when SIINFEKL is genetically appended to the predicted C terminus of NEG8, as shown by activation of OT-I T cells in vitro and in vivo. Moreover, recombinant IAV encoding of SIINFEKL embedded in the negative strand of the neuraminidase-stalk coding sequence also activates OT-I T cells in mice. Together, our findings demonstrate both the translation of sequences on the negative strand of a single-stranded RNA virus and its relevance in antiviral immunosurveillance.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
/
ARN Viral
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Linfocitos T CD8-positivos
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Vigilancia Inmunológica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Immunol
Año:
2018
Tipo del documento:
Article