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Low irradiance multiphoton imaging with alloyed lanthanide nanocrystals.
Tian, Bining; Fernandez-Bravo, Angel; Najafiaghdam, Hossein; Torquato, Nicole A; Altoe, M Virginia P; Teitelboim, Ayelet; Tajon, Cheryl A; Tian, Yue; Borys, Nicholas J; Barnard, Edward S; Anwar, Mekhail; Chan, Emory M; Schuck, P James; Cohen, Bruce E.
Afiliación
  • Tian B; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Fernandez-Bravo A; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Najafiaghdam H; Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, Berkeley, CA, 94720, USA.
  • Torquato NA; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Altoe MVP; Department of Radiation Oncology, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Teitelboim A; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Tajon CA; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Tian Y; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Borys NJ; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Barnard ES; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Anwar M; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Chan EM; Department of Radiation Oncology, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Schuck PJ; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. emchan@lbl.gov.
  • Cohen BE; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. p.j.schuck@columbia.edu.
Nat Commun ; 9(1): 3082, 2018 08 06.
Article en En | MEDLINE | ID: mdl-30082844
ABSTRACT
Multiphoton imaging techniques that convert low-energy excitation to higher energy emission are widely used to improve signal over background, reduce scatter, and limit photodamage. Lanthanide-doped upconverting nanoparticles (UCNPs) are among the most efficient multiphoton probes, but even UCNPs with optimized lanthanide dopant levels require laser intensities that may be problematic. Here, we develop protein-sized, alloyed UCNPs (aUCNPs) that can be imaged individually at laser intensities >300-fold lower than needed for comparably sized doped UCNPs. Using single UCNP characterization and kinetic modeling, we find that addition of inert shells changes optimal lanthanide content from Yb3+, Er3+-doped NaYF4 nanocrystals to fully alloyed compositions. At high levels, emitter Er3+ ions can adopt a second role to enhance aUCNP absorption cross-section by desaturating sensitizer Yb3+ or by absorbing photons directly. Core/shell aUCNPs 12 nm in total diameter can be imaged through deep tissue in live mice using a laser intensity of 0.1 W cm-2.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos