Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.
Nat Methods
; 15(9): 685-688, 2018 09.
Article
en En
| MEDLINE
| ID: mdl-30127504
Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Aptámeros de Nucleótidos
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania