Efficient purification of a recombinant tag-free thermostable <i>Kluyveromyces marxianus</i> uricase by pH-induced self-cleavage of intein and expression in <i>Escherichia coli</i>.

Wang, Bangchun; Luo, Laipeng; Wang, Dongmei; Ding, Rui; Hong, Jiong

Efficient purification of a recombinant tag-free thermostable Kluyveromyces marxianus uricase by pH-induced self-cleavage of intein and expression in Escherichia coli.

Wang, Bangchun; Luo, Laipeng; Wang, Dongmei; Ding, Rui; Hong, Jiong.

Afiliación

Wang B; 1Institutes of Life Science, Anhui Medical University, No. 81 Mei Shan Road, Hefei, 230032 Anhui China.
Luo L; 1Institutes of Life Science, Anhui Medical University, No. 81 Mei Shan Road, Hefei, 230032 Anhui China.
Wang D; 2School of Life Science, University of Science and Technology of China, No. 96 Jinzhai Road, Hefei, 230036 Anhui China.
Ding R; 1Institutes of Life Science, Anhui Medical University, No. 81 Mei Shan Road, Hefei, 230032 Anhui China.
Hong J; 2School of Life Science, University of Science and Technology of China, No. 96 Jinzhai Road, Hefei, 230036 Anhui China.

3 Biotech ; 8(9): 400, 2018 Sep.

Article en En | MEDLINE | ID: mdl-30221113