miR-342-3p promotes osteogenic differentiation via targeting ATF3.
FEBS Lett
; 592(24): 4051-4065, 2018 12.
Article
en En
| MEDLINE
| ID: mdl-30381822
ABSTRACT
Through their multiple targets, microRNAs (miRNAs) are involved in numerous physiological and pathological processes. In this study, miR-342-3p was found to be deregulated with ossification of ligament or osteoporosis. We demonstrate that silencing miR-342-3p impairs osteoblast activity and matrix mineralization, while over expression of miR-342-3p promotes osteoblast differentiation significantly. Moreover, miR-342-3p directly targets activating transcription factor 3 (ATF3), which inhibits transcription of pro-osteogenic differentiation-associated genes. In addition, there exists a higher frequency of methylation at the CpG island of the Enah/Vasp-Like (EVL) locus in undifferentiated pre-osteoblasts; however, demethylation of the EVL CpG island induces over expression of miR-342-3p during osteogenic differentiation. This study suggests that miR-342-3p may serves as a potential marker for diagnosis and treatment of ossification of ligament and osteoporosis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Osteoblastos
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Osteogénesis
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Diferenciación Celular
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MicroARNs
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Factor de Transcripción Activador 3
Límite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
FEBS Lett
Año:
2018
Tipo del documento:
Article
País de afiliación:
China