A rapid and robust method for single cell chromatin accessibility profiling.
Nat Commun
; 9(1): 5345, 2018 12 17.
Article
en En
| MEDLINE
| ID: mdl-30559361
ABSTRACT
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, very few studies have been performed at the single cell level (scATAC-seq) due to technical challenges. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk Tn5 tagging with single-nuclei sorting. We demonstrate that our method works robustly across various systems, including fresh and cryopreserved cells from primary tissues. By profiling over 3000 splenocytes, we identify distinct immune cell types and reveal cell type-specific regulatory regions and related transcription factors.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cromatina
/
Análisis de Secuencia de ADN
/
Transposasas
/
Análisis de la Célula Individual
/
Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Reino Unido