Next-Generation Sequencing Accelerates Crop Gene Discovery.
Trends Plant Sci
; 24(3): 263-274, 2019 03.
Article
en En
| MEDLINE
| ID: mdl-30573308
ABSTRACT
The identification and isolation of genes underlying quantitative trait loci (QTLs) associated with agronomic traits in crops have been recently accelerated thanks to next-generation sequencing (NGS)-based technologies combined with plant genetics. With NGS, various revisited genetic approaches, which benefited from higher marker density, have been elaborated. These approaches improved resolution in QTL position and assisted in determining functional causative variations in genes. Examples of QTLs/genes associated with agronomic traits in crops and identified using different strategies based on whole-genome sequencing (WGS)/whole-genome resequencing (WGR) or RNA-seq are presented and discussed in this review. More specifically, we summarize and illustrate how NGS boosted bulk-segregant analysis (BSA), expression profiling, and the construction of polymorphism databases to facilitate the detection of QTLs and causative genes.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sitios de Carácter Cuantitativo
/
Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Trends Plant Sci
Asunto de la revista:
BOTANICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Vietnam