Validation of spectrophotometric determination of chlorogenic acid in fermentation broth and fruits.
Food Chem
; 278: 170-177, 2019 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-30583358
ABSTRACT
A fast and accurate ultraviolet-visible (UV-vis) spectrophotometric method was established to determine the presence of chlorogenic acid (CGA) according to potassium ferricyanide-Fe (III) detection system. The reaction temperature and pH level greatly influenced the CGA absorption spectrum. Maximum UV-vis absorption wavelength occurred at 790â¯nm under the optimum conditions (at 25⯰C and pH 7.0). The results of UV-vis were further assessed by comparing the results with those of high-performance liquid chromatography (HPLC). The proposed method showed a wide linear sensing range of 10.0-800.0⯵gâ¯mL-1 (R2â¯=â¯0.9996) and a high degree of precision (%R.S.D.â¯<â¯1.50) and recovery (%R.S.D.â¯>â¯3.39). Results of CGA and HPLC methods correlated well with each other. The proposed accurate, rapid, sensitive, low-cost, and high-throughput method was successfully used to quantify CGA in fermentation broth and fruits (Mango). Therefore, it may be applied for measuring CGA in biological samples.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrofotometría Ultravioleta
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Ácido Clorogénico
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Análisis de los Alimentos
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Frutas
Idioma:
En
Revista:
Food Chem
Año:
2019
Tipo del documento:
Article
País de afiliación:
China