Transcriptomic and physiological changes in western mosquitofish (Gambusia affinis) after exposure to norgestrel.
Ecotoxicol Environ Saf
; 171: 579-586, 2019 Apr 30.
Article
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| MEDLINE
| ID: mdl-30654292
ABSTRACT
Norgestrel (NGT) is a synthetic progestin used in human and veterinary medicine. Adult female mosquitofish were exposed to NGT for 42 d at 377â¯ngâ¯L-1. The fin morphology and the liver transcriptome were assessed. NGT exposure increased ray 46 length ratio. As compared to the control, NGT treatment affected the expression of 11,772 annotated transcripts in female mosquitofish. Specifically, we found 5780 were repressed while 5992 were significantly induced. Gene ontology (GO) analysis showed that 53 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways and 158 GO terms were significantly over expressed. Genes showing the largest magnitude of expression changes were related to fin development, androgen biosynthesis, and lipid and fatty acid metabolisms, suggesting the involvement of these biological processes in response to NGT exposure in G. affinis. This first comprehensive study on the transcriptomic alterations by NGT in G. affinis not only provides valuable information on the development of molecular markers but also opens new avenues for studies on the molecular mechanisms of effects of NGT in particular and possibly other progestins in G. affinis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ciprinodontiformes
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Transcriptoma
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Hígado
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Norgestrel
Límite:
Animals
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2019
Tipo del documento:
Article
País de afiliación:
China