Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples.
Braz J Med Biol Res
; 52(2): e8194, 2019 Feb 14.
Article
en En
| MEDLINE
| ID: mdl-30785480
ABSTRACT
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
/
Síndromes Mielodisplásicos
/
Células de la Médula Ósea
/
Leucemia Mieloide
/
Cariotipificación
/
Trastornos Mieloproliferativos
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Braz J Med Biol Res
Año:
2019
Tipo del documento:
Article
País de afiliación:
Brasil