Long-term self-renewal of naïve neural stem cells in a defined condition.
Biochim Biophys Acta Mol Cell Res
; 1866(6): 971-977, 2019 06.
Article
en En
| MEDLINE
| ID: mdl-30857870
ABSTRACT
During embryonic development, neural stem cells (NSCs) emerge as early as the neural plate stage and give rise to the nervous system. Early-stage NSCs express Sry-related-HMG box-1 (Sox1) and are biased towards neuronal differentiation. However, long-term maintenance of early-stage NSCs in vitro remains a challenge. Here, we report development of a defined culture condition for the long-term maintenance of Sox1-positive early-stage mouse NSCs. The proliferative ability of these Sox1-positive NSCs was confirmed by clonal propagation. Compared to the NSCs cultured using the traditional culture condition, the long-term self-renewing Sox1-positive NSCs efficiently differentiate into neurons and exhibit an identity representative of the anterior and midbrain regions. These early-stage Sox1-positive NSCs could also be switched to late-stage NSCs by being cultured with bFGF/EGF, which can then differentiate into astrocytes and oligodendrocytes. The long-term self-renewing Sox1-positive NSCs were defined as naïve NSCs, based on their high neuronal differentiation capacity and anterior regional identity. This culture condition provides a robust platform for further dissection of the NSC self-renewal mechanism and promotes potential applications of NSCs for cell-based therapy on nervous system disorders.
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Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
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Péptidos y Proteínas de Señalización Intercelular
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Bibliotecas de Moléculas Pequeñas
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Células-Madre Neurales
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta Mol Cell Res
Año:
2019
Tipo del documento:
Article