Soluble siglec-5 is a novel salivary biomarker for primary Sjogren's syndrome.
J Autoimmun
; 100: 114-119, 2019 06.
Article
en En
| MEDLINE
| ID: mdl-30922727
ABSTRACT
Despite advances in the understanding of the pathogenesis, disease-specific biomarkers have not been included in the classification criteria for Primary Sjogren's syndrome (pSS). Based on a microarray of peripheral blood mononuclear cells (PBMCs) from patients with primary Sjogren's syndrome (pSS), we aimed to investigate whether soluble sialic acid-binding immunoglobulin-like lectin (siglec)-5 in saliva might be a biomarker for pSS. The concentration of siglec-5 in saliva and sera was determined by ELISA. Clinical parameters related with pSS were obtained from pSS registry and correlation with salivary siglec-5 level was evaluated. Receiver operating curve (ROC) analysis was performed to determine cut off value. A separate validation cohort consisted of subjects with suspicious pSS was evaluated to determine the performance. The level of salivary siglec-5 was significantly higher in pSS patients (nâ¯=â¯170) compared with HCs (nâ¯=â¯25), non SS sicca patients (nâ¯=â¯78) or patients with systemic lupus erythematosus (SLE) (nâ¯=â¯43) (1346.8 [202.8-4280.0] pg/mL, 6.08 [0-134.0] pg/mL, 195 [0-947.5] pg/mL, and 0 [0-238.7] pg/mL, median [interquartile range], Pâ¯<â¯0.001). Salivary siglec-5 level negatively correlated with salivary flow rate (spearman's rho -0.420, Pâ¯<â¯0.001), and positively correlated with ocular surface score (rho 0.331, Pâ¯<â¯0.001) and serum immunoglobulin G level (rhoâ¯=â¯0.202, Pâ¯=â¯0.008). In ROC analysis, area under the curve was 0.774[0.724-0.826]. With a cut off value of 400â¯pg/mL, sensitivity and specificity was 0.69 and 0.70 respectively. In validation cohort (45â¯pSS patients and 45 non SS sicca patients), sensitivity and specificity of siglec-5 was 64.4% and 77.8%, respectively. In conclusion, the level of soluble siglec-5 is significantly higher in the saliva from pSS patients, which reflects the severity of hyposalivation and ocular surface damage. This novel salivary biomarker may provide benefits for pSS diagnosis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Saliva
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Antígenos de Diferenciación Mielomonocítica
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Antígenos CD
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Lectinas
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Autoimmun
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2019
Tipo del documento:
Article