Tissue Handling and Dissociation for Single-Cell RNA-Seq.
Methods Mol Biol
; 1979: 9-21, 2019.
Article
en En
| MEDLINE
| ID: mdl-31028629
ABSTRACT
The starting material for all single-cell protocols is a cell suspension. The particular functions and spatial distribution of immune cells generally make them easy to isolate them from the tissues where they dwell. Here we describe tissue dissociation protocols that have been used to obtain human immune cells from lymphoid and nonlymphoid tissues to be then used as input to single-cell methods. We highlight the main factors that can influence the final quality of single-cell data, namely the stress signatures that can bias its interpretation.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
/
ARN
/
Análisis de Secuencia de ARN
/
Perfilación de la Expresión Génica
/
Análisis de la Célula Individual
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido