Lipid Analysis of 30â¯000 Individual Rodent Cerebellar Cells Using High-Resolution Mass Spectrometry.
Anal Chem
; 91(12): 7871-7878, 2019 06 18.
Article
en En
| MEDLINE
| ID: mdl-31122012
ABSTRACT
Single-cell measurements aid our understanding of chemically heterogeneous systems such as the brain. Lipids are one of the least studied chemical classes, and their cell-to-cell heterogeneity remains largely unexplored. We adapted microscopy-guided single-cell profiling using matrix-assisted laser desorption/ionization ion cyclotron resonance mass spectrometry to profile the lipid composition of over 30â¯000 individual rat cerebellar cells. We detected 520 lipid features, many of which were found in subsets of cells; Louvain clustering identified 101 distinct groups that can be correlated to neuronal and astrocytic classifications and lipid classes. Overall, the two most common lipids found were [PC(320)+H]+ and [PC(341)+H]+, which were present within 98.9 and 89.5% of cells, respectively; lipid signals present in <1% of cells were also detected, including [PC(341)+K]+ and [PG(402(OH))+Na]+. These results illustrate the vast lipid heterogeneity found within rodent cerebellar cells and hint at the distinct functional consequences of this heterogeneity.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cerebelo
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Análisis de la Célula Individual
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Lípidos
Límite:
Animals
Idioma:
En
Revista:
Anal Chem
Año:
2019
Tipo del documento:
Article