Proteomic profile of follicular fluid from patients with polycystic ovary syndrome (PCOS) submitted to in vitro fertilization (IVF) compared to oocyte donors.
JBRA Assist Reprod
; 23(4): 367-391, 2019 10 14.
Article
en En
| MEDLINE
| ID: mdl-31173497
ABSTRACT
OBJECTIVE:
The follicular fluid (FF) of women with polycystic ovary syndrome (PCOS) seems to exhibit a profile different from that of fertile women, which may be related to folliculogenesis disruption in PCOS patients. The aim of this study was to evaluate the differentially expressed proteins in the FF of women with PCOS compared to oocyte donors (ODs).METHODS:
This screening study included thirteen (13) women who underwent in vitro fertilization (IVF) cycles seven (7) ODs and six (6) PCOS patients. The patients underwent standard ovarian stimulation, and the FF was analysed using ion trap and time-of-flight liquid chromatography-mass spectrometry (LCMS-IT-TOF).RESULTS:
The FF of the patients was matched to 229 proteins, with 61 proteins exclusive to the PCOS group, 123 proteins exclusive to the ODs, and 45 proteins found in both groups. We highlight fetuin-A and vitamin D ligand protein, which were exclusively expressed in the PCOS group; Complement C3 overexpressed in the PCOS group; and 26S protease only expressed in the OD group. The canonical pathways LXR/RXR activation, FXR/RXR activation, prothrombin activation are directly related to the disrupted metabolism and increased inflammatory status found in PCOS patients.CONCLUSIONS:
The findings of the differentially expressed proteins and matched pathways are associated with folliculogenesis, indicating it relevance to oocyte quality.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Síndrome del Ovario Poliquístico
/
Fertilización In Vitro
/
Líquido Folicular
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Adult
/
Female
/
Humans
Idioma:
En
Revista:
JBRA Assist Reprod
Año:
2019
Tipo del documento:
Article
País de afiliación:
Brasil