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Extracellular matrix derived by human umbilical cord-deposited mesenchymal stem cells accelerates chondrocyte proliferation and differentiation potential in vitro.
Zhang, Weixiang; Yang, Jianhua; Zhu, Yun; Sun, Xun; Guo, Weimin; Liu, Xuejian; Jing, Xiaoguang; Guo, Ganggang; Guo, Quanyi; Peng, Jiang; Zhu, Xiaofeng.
Afiliación
  • Zhang W; The People's Hospital of Lanxi, 1359th Shanxi Road, Lanxi, 321100, China.
  • Yang J; Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China.
  • Zhu Y; School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China.
  • Sun X; School of Biomedical Sciences, The University of Hong Kong, 21 Sassoon Road, Pokfulam, 999777, Hong Kong.
  • Guo W; Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China.
  • Liu X; School of Medicine, Nankai University, 94th Weijin Road, Nankai District, Tianjin, 300071, China.
  • Jing X; Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China.
  • Guo G; School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China.
  • Guo Q; Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China.
  • Peng J; Zhengzhou Yihe Hospital Affiliated to Henan University, 69th Nongyedong Road, Zhengzhou, 450000, China.
  • Zhu X; School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China.
Cell Tissue Bank ; 20(3): 351-365, 2019 Sep.
Article en En | MEDLINE | ID: mdl-31218457
The extracellular matrix (ECM) is a dynamic and intricate three-dimensional (3D) microenvironment with excellent biophysical, biomechanical, and biochemical properties that may directly or indirectly regulate cell behavior, including proliferation, adhesion, migration, and differentiation. Compared with tissue-derived ECM, cell-derived ECM potentially has more advantages, including less potential for pathogen transfer, fewer inflammatory or anti-host immune responses, and a closer resemblance to the native ECM microenvironment. Different types of cell-derived ECM, such as adipose stem cells, synovium-derived stem cells and bone marrow stromal cells, their effects on articular chondrocytes which have been researched. In this study, we aimed to develop a 3D cell culture substrate using decellularized ECM derived from human umbilical cord-derived mesenchymal stem cells (hUCMSCs), and evaluated the effects on articular chondrocytes. We evaluated the morphology and components of hUCMSC-derived ECM using physical and chemical methods. Morphological, histological, immunohistochemical, biochemical, and real-time PCR analyses demonstrated that proliferation and differentiation capacity of chondrocytes using the 3D hUCMSC-derived ECM culture substrate was superior to that using non-coated two-dimensional plastic culture plates. In conclusion, 3D decellularized ECM derived from hUCMSCs offers a tissue-specific microenvironment for in vitro culture of chondrocytes, which not only markedly promoted chondrocyte proliferation but also preserved the differentiation capacity of chondrocytes. Therefore, our findings suggest that a 3D cell-derived ECM microenvironment represents a promising prospect for autologous chondrocyte-based cartilage tissue engineering and regeneration. The hUCMSC-derived ECM as a biomaterial is used for the preparation of scaffold or hybrid scaffold products which need to further study in the future.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cordón Umbilical / Diferenciación Celular / Condrocitos / Proliferación Celular / Matriz Extracelular / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2019 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cordón Umbilical / Diferenciación Celular / Condrocitos / Proliferación Celular / Matriz Extracelular / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2019 Tipo del documento: Article País de afiliación: China