Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting.

Son, Francheska; Umphred-Wilson, Katharine; Shim, Jae-Hyuck; Adoro, Stanley

Son, Francheska; Umphred-Wilson, Katharine; Shim, Jae-Hyuck; Adoro, Stanley.

Afiliación

Son F; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Umphred-Wilson K; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Shim JH; Division of Rheumatology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Adoro S; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA. sxa726@case.edu.

Methods Mol Biol ; 1998: 219-226, 2019.

Article en En | MEDLINE | ID: mdl-31250305

Asunto(s)

Western Blotting/métodos; Complejos de Clasificación Endosomal Requeridos para el Transporte/metabolismo; Inmunoprecipitación/métodos; Ubiquitinación; Endosomas/metabolismo; Células HEK293; Humanos; Células Jurkat; Linfocitos T

Palabras clave

CHMP5; Deubiquitinase; Posttranslational modification; T lymphocytes; Ubiquitination

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Western Blotting / Inmunoprecipitación / Ubiquitinación / Complejos de Clasificación Endosomal Requeridos para el Transporte Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google