Chloroquine inhibits endosomal viral RNA release and autophagy-dependent viral replication and effectively prevents maternal to fetal transmission of Zika virus.
Antiviral Res
; 169: 104547, 2019 09.
Article
en En
| MEDLINE
| ID: mdl-31251958
ABSTRACT
Zika virus (ZIKV) infection can cause neonatal microcephaly and neurological disorders. Currently, there is no designated drug for treating ZIKV infection and preventing neonatal microcephaly. In this study, we evaluated the effect of chloroquine, an anti-malaria drug, in ZIKV infected cells and mouse models. Chloroquine significantly inhibited ZIKV infection in multiple mammalian cell lines. Chloroquine treatment significantly improved the survival of ZIKV-infected 1-day old suckling SCID Beige mice and reduced viremia in adult SCID Beige mice. Importantly, chloroquine protected the fetus from maternal infection by reducing placenta to fetus viral transmission. We found that chloroquine exerts at least two mechanisms in protecting against ZIKV infection 1) inhibiting endosomal disassembly of the internalized virus and thus reducing the release of viral RNA to the cytoplasm for replication; 2) inhibiting ZIKV RNA replication through blocking ZIKV induced autophagy. Our study suggests that chloroquine treatment warrants to be considered as a mitigation strategy for treating ZIKV infection and preventing ZIKV-associated microcephaly in pregnant women.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Endosomas
/
Autofagia
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Replicación Viral
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ARN Viral
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Cloroquina
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Virus Zika
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Infección por el Virus Zika
Límite:
Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Antiviral Res
Año:
2019
Tipo del documento:
Article
País de afiliación:
China