Ruegeria lutea sp. nov., isolated from marine sediment, Masan Bay, South Korea.
Int J Syst Evol Microbiol
; 69(9): 2854-2861, 2019 Sep.
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| MEDLINE
| ID: mdl-31274408
ABSTRACT
A Gram-stain-negative, non-motile, mesophilic, short rod-shaped, aerobic bacterium designated as 318-1T was isolated from a marine sediment collected from Masan Bay, South Korea. Strain 318-1T grew optimally at pH 6-7, at 30 °C and in the presence of 2-3â% (w/v) NaCl, tolerant of up to 8â% (w/v) NaCl, and accumulated poly-ß-hydroxybutyrate (PHB). A comparative analysis of 16S rRNA gene sequences revealed that strain 318-1T formed a distinct phyletic lineage in the genus Ruegeria (family Rhodobacteraceae, class Alphaproteobacteria) and showed high sequence similarity to Ruegeria halocynthiae DSM 27839T (96.5â%) and Shimia haliotis DSM 28453T (96.3â%). Comparing the genome sequence of 318-1T with those of the type strains of seven species of the genus Rugeria and two species of the genus Shimia, the values obtained were below the thresholds with analysis of average nucleotide identities (ANI, 71.6-76.8â%) and in silico DNA-DNA hybridisation, Genome-to-Genome Distance Calculator (GGDC, 18.5-20.6â%). The DNA G+C content was 65.75 mol%. Chemotaxonomic data [predominant quinone ubiquinone Q10; polar lipid profile consisting of major compounds phosphatidylcholine (PC), phosphatidylglycerol (PG), an unidentified aminolipid and an unidentified lipid; major fatty acids summed feature 8 (C18ââ1ω7c and/or C18ââ1ω6c)] supported the affiliation of strain 318-1T to the genus Ruegeria. Genomic, chemotaxonomic, and phenotypic differentiation of strain 318-1T from the members of the genus Ruegeria support it as a novel species. On the basis of the results in this study, a novel species, Ruegeria lutea sp. nov., is proposed. The type strain is 318-1T (=JCM 30927T=KEMB 7306-525T=KCTC 72105T).
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Banco de datos:
MEDLINE
Asunto principal:
Filogenia
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Agua de Mar
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Sedimentos Geológicos
/
Rhodobacteraceae
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2019
Tipo del documento:
Article