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Modulators of the endocannabinoid system influence skin barrier repair, epidermal proliferation, differentiation and inflammation in a mouse model.
Proksch, Ehrhardt; Soeberdt, Michael; Neumann, Claudia; Kilic, Ana; Abels, Christoph.
Afiliación
  • Proksch E; Department of Dermatology, University of Kiel, Kiel, Germany.
  • Soeberdt M; Dr. August Wolff GmbH & Co. KG, Bielefeld, Germany.
  • Neumann C; Department of Dermatology, University of Kiel, Kiel, Germany.
  • Kilic A; Dr. August Wolff GmbH & Co. KG, Bielefeld, Germany.
  • Abels C; Dr. August Wolff GmbH & Co. KG, Bielefeld, Germany.
Exp Dermatol ; 28(9): 1058-1065, 2019 09.
Article en En | MEDLINE | ID: mdl-31350927
Endocannabinoids (ECs) are important regulators of cell signalling. Cannabinoid receptors are involved in keratinocyte proliferation/differentiation. Elevation of the endogenous cannabinoid tone leads to strong anti-inflammatory effects. Here, we explored the influence of endocannabinoid system (ECS) modulators on skin permeability barrier repair, epidermal proliferation, differentiation and inflammation in hairless mice. We used WOBE440, a selective fatty acid amide hydrolase (FAAH) inhibitor, WOL067-531, an inhibitor of endocannabinoid reuptake with no relevant FAAH activity, which both signal via cannabinoid receptor-1 and cannabinoid receptor-2 (CB-1R and CB-2R) and compared them to WOBE15 which signals via CB-2R. Barrier disruption and skin irritation were induced by tape stripping or by sodium dodecyl sulphate (SDS) patch testing. Immediately after barrier disruption, 30 µL of 0.5% WOBE440, WOL067-531 and WOBE15 solutions or the vehicle was applied topically. Barrier repair was monitored by transepidermal water loss at 1.5, 3, 5 and 7 hours. We found that barrier repair was significantly delayed by WOL067-531. A tendency for a delay was noticed for WOBE440, whereas for WOBE15, no effect was observed. Immunohistology showed that the tape-stripping-induced increase in epidermal proliferation and filaggrin expression was significantly reduced by topical applications of WOL067-531 and WOBE440, but not by WOBE15. Also, the SDS-induced inflammation, as determined by the number of inflammatory cells, was reduced by WOL067-531 and WOBE440. In summary, we showed that WOL067-531 exhibits a significant effect on skin barrier repair, epidermal proliferation/differentiation and inflammation.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Absorción Cutánea / Endocannabinoides Límite: Animals Idioma: En Revista: Exp Dermatol Asunto de la revista: DERMATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Absorción Cutánea / Endocannabinoides Límite: Animals Idioma: En Revista: Exp Dermatol Asunto de la revista: DERMATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania