Constraints to counting bioluminescence producing cells by a commonly used transgene promoter and its implications for experimental design.

Mosaad, E O; Futrega, K; Seim, I; Gloss, B; Chambers, K F; Clements, J A; Doran, M R

Mosaad, E O; Futrega, K; Seim, I; Gloss, B; Chambers, K F; Clements, J A; Doran, M R.

Afiliación

Mosaad EO; School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
Futrega K; Australian Prostate Cancer Research Centre-Queensland (APCRC-Q), Translational Research Institute, Brisbane, Australia.
Seim I; Biochemistry Division, Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt.
Gloss B; School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
Chambers KF; Australian Prostate Cancer Research Centre-Queensland (APCRC-Q), Translational Research Institute, Brisbane, Australia.
Clements JA; Comparative and Endocrine Biology Laboratory, Translational Research Institute-Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia.
Doran MR; Integrative Biology Laboratory, College of Life Sciences, Nanjing Normal University, Nanjing, China.

Sci Rep ; 9(1): 11334, 2019 08 05.

Article en En | MEDLINE | ID: mdl-31383876

Asunto(s)

Luciferasas/aislamiento & purificación; Mediciones Luminiscentes/métodos; Proteínas Luminiscentes/aislamiento & purificación; Células Madre Mesenquimatosas/metabolismo; Línea Celular Tumoral; Técnicas de Cocultivo; Regulación Neoplásica de la Expresión Génica/genética; Genes Reporteros/genética; Humanos; Luciferasas/química; Proteínas Luminiscentes/química; Masculino; Células Madre Mesenquimatosas/patología; Regiones Promotoras Genéticas/genética; Neoplasias de la Próstata/genética; Neoplasias de la Próstata/patología; Transgenes/genética

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Luciferasas / Mediciones Luminiscentes / Proteínas Luminiscentes Límite: Humans / Male Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Australia

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