Cost-Effective Profiling of Mutator Transposon Insertions in Maize by Next-Generation Sequencing.
Methods Mol Biol
; 2072: 39-50, 2020.
Article
en En
| MEDLINE
| ID: mdl-31541437
ABSTRACT
Transposable elements can be highly mutagenic because when they transpose they can insert into genes and disrupt their function, a propensity which has been exploited in many organisms to generate tagged mutant alleles. The Mutator (Mu) family transposon is a family of DNA-type transposons in maize with a particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active Mu elements. Here we describe a rapid and cost-effective Miseq-based Mu transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Análisis Mutacional de ADN
/
Elementos Transponibles de ADN
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Mutagénesis Insercional
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Zea mays
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Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Health_economic_evaluation
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos