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Efficient Biosynthesis of High-Value Succinic Acid and 5-Hydroxyleucine Using a Multienzyme Cascade and Whole-Cell Catalysis.
Sun, Dengyue; Liu, Xin; Zhu, Menglu; Chen, Ying; Li, Chao; Cheng, Xiaotao; Zhu, Zhangliang; Lu, Fuping; Qin, Hui-Min.
Afiliación
  • Sun D; Key Laboratory of Industrial Fermentation Microbiology , Ministry of Education , Tianjin 300457 , People's Republic of China.
  • Liu X; College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , People's Republic of China.
  • Zhu M; Tianjin Key Laboratory of Industrial Microbiology , Tianjin 300457 , People's Republic of China.
  • Chen Y; College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , People's Republic of China.
  • Li C; College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , People's Republic of China.
  • Cheng X; College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , People's Republic of China.
  • Zhu Z; College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , People's Republic of China.
  • Lu F; College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , People's Republic of China.
  • Qin HM; Key Laboratory of Industrial Fermentation Microbiology , Ministry of Education , Tianjin 300457 , People's Republic of China.
J Agric Food Chem ; 67(45): 12502-12510, 2019 Nov 13.
Article en En | MEDLINE | ID: mdl-31623431
Succinic acid (SA) is applied in the food, chemical, and pharmaceutical industries. 5-Hydroxyleucine (5-HLeu) is a promising precursor for the biosynthesis of antituberculosis drugs. Here, we designed a promising synthetic route for the simultaneous production of SA and 5-HLeu by combining l-leucine dioxygenase (NpLDO), l-glutamate oxidase (LGOX), and catalase (CAT). Two bioconversion systems: "a multienzyme cascade catalysis in vitro" (MECCS) and "whole-cell catalysis system" (WCCS) were constructed. A high-activity NpLDO mutant was screened by error-prone polymerase chain reaction (PCR) and showed 6.1-fold improvement of catalytic activity. After optimization of reaction conditions, MECSS yielded 3.15 g/L SA and 3.92 g/L 5-HLeu, while the production of SA and 5-HLeu by the most effective WCSS reached 15.12 and 18.83 g/L, respectively. This is the first attempt to use ferrous iron/α-ketoglutarate-dependent dioxygenases for the simultaneous production of SA and hydroxy-amino-acid. This research provides a tool for industrial production of food of high-value products from low-cost raw materials.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Catalasa / Ácido Succínico / Nostoc / Dioxigenasas / Aminoácido Oxidorreductasas / Leucina Idioma: En Revista: J Agric Food Chem Año: 2019 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Catalasa / Ácido Succínico / Nostoc / Dioxigenasas / Aminoácido Oxidorreductasas / Leucina Idioma: En Revista: J Agric Food Chem Año: 2019 Tipo del documento: Article