Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes.
Head Face Med
; 15(1): 27, 2019 Nov 11.
Article
en En
| MEDLINE
| ID: mdl-31711509
ABSTRACT
BACKGROUND:
Controlled release of proteins bound to conventional bone substitutes is still insufficient. Therefore, this study evaluates in-vitro release kinetics of the model protein FITC-BSA (fluorescein conjugated bovine serum albumine) from insoluble bovine collagenous bone matrices (ICBM) with different polymer coatings. Analyzes aim at comparing FITC-BSA release from uncoated versus coated ICBM over time to find bone substitute coatings with consistent release profiles.METHODS:
Release kinetics of FITC-BSA from uncoated as well as coated ICBM with five different polymers (RESOMER R 203 H, RG 503 H, RG 504 H, RG 505, L 206 S) were measured over a period of 11 days (d). Measurements were conducted after 6 h (h), 12 h, 24 h, 3 d, 5 d, 7 d, 9 d and 11 d with six samples for each coated ICBM. Two groups were formed (1) with and (2) without medium change at times of measurement. For each group ANOVA with post-hoc Bonferroni testing was used. Scanning electron microscopy assessed morphologic differences between ICBM coating.RESULTS:
In group 1 approx. 70% of FITC-BSA release from uncoated ICBM occurred after 6 h compared to approx. 50% in group 2. Only polymers with medium inherent viscosity, i.e. RESOMER RG 503 H, constantly showed significantly more FITC-BSA release throughout 11 d than uncoated ICBM (p = 0.007). The same was found for group 2 (p = 0.005). No significant differences between PLA and PLGA polymers were found. Scanning electron microscopy results indicate a weak adhesion of polymer coatings to ICBM explaining its rather weak retentive effect on overall FITC-BSA release.CONCLUSIONS:
Medium molecular size polymers reduce the overall released FITC-BSA from ICBM over time. In clinical practice these polymers may prove ideal for bone substitute materials.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Albúmina Sérica Bovina
/
Fluoresceína-5-Isotiocianato
/
Sustitutos de Huesos
Límite:
Animals
Idioma:
En
Revista:
Head Face Med
Asunto de la revista:
MEDICINA
/
ODONTOLOGIA
/
ORTOPEDIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Alemania