Macrophages protect mycoplasma-infected chronic myeloid leukemia cells from natural killer cell killing.
Immunol Cell Biol
; 98(2): 138-151, 2020 02.
Article
en En
| MEDLINE
| ID: mdl-31837284
ABSTRACT
Macrophages (MÏ) have been reported to downmodulate the cytotoxicity of natural killer (NK) cell against solid tumor cells. However, the collaborative role between NK cells and MÏ remains underappreciated, especially in hematological cancers, such as chronic myeloid leukemia (CML). We observed a higher ratio of innate immune cells (MÏ and NK) to adaptive immune cells (T and B cells) in CML bone marrow aspirates, prompting us to investigate the roles of NK and MÏ in CML. Using coculture models simulating the tumor inflammatory environment, we observed that MÏ protects CML from NK attack only when CML was itself mycoplasma-infected and under chronic infection-inflammation condition. We found that the MÏ-protective effect on CML was associated with the maintenance of CD16 level on the NK cell membrane. Although the NK membrane CD16 (mCD16) was actively shed in MÏ + NK + CML trioculture, the NK mCD16 level was maintained, and this was independent of the modulation of sheddase by tissue inhibitor of metalloproteinase 1 or inhibitory cytokine transforming growth factor beta. Instead, we found that this process of NK mCD16 maintenance was conferred by MÏ in a contact-dependent manner. We propose a new perspective on anti-CML strategy through abrogating MÏ-mediated retention of NK surface CD16.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Asesinas Naturales
/
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Inflamación
/
Macrófagos
/
Mycoplasma
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Immunol Cell Biol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Singapur