Conformal Coating of Stem Cell-Derived Islets for ß Cell Replacement in Type 1 Diabetes.
Stem Cell Reports
; 14(1): 91-104, 2020 01 14.
Article
en En
| MEDLINE
| ID: mdl-31839542
ABSTRACT
The scarcity of donors and need for immunosuppression limit pancreatic islet transplantation to a few patients with labile type 1 diabetes. Transplantation of encapsulated stem cell-derived islets (SC islets) might extend the applicability of islet transplantation to a larger cohort of patients. Transplantation of conformal-coated islets into a confined well-vascularized site allows long-term diabetes reversal in fully MHC-mismatched diabetic mice without immunosuppression. Here, we demonstrated that human SC islets reaggregated from cryopreserved cells display glucose-stimulated insulin secretion in vitro. Importantly, we showed that conformally coated SC islets displayed comparable in vitro function with unencapsulated SC islets, with conformal coating permitting physiological insulin secretion. Transplantation of SC islets into the gonadal fat pad of diabetic NOD-scid mice revealed that both unencapsulated and conformal-coated SC islets could reverse diabetes and maintain human-level euglycemia for more than 80 days. Overall, these results provide support for further evaluation of safety and efficacy of conformal-coated SC islets in larger species.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Diferenciación Celular
/
Trasplante de Islotes Pancreáticos
/
Diabetes Mellitus Tipo 1
/
Células Secretoras de Insulina
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Stem Cell Reports
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos