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Development and Characterization of a Porcine Liver Scaffold.
Ansari, Tahera; Southgate, Aaron; Obiri-Yeboa, Irene; Jones, Lauren G; Greco, Karin; Olayanju, Adedamola; Mbundi, Lubinda; Somasundaram, Murali; Davidson, Brian; Sibbons, Paul D.
Afiliación
  • Ansari T; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Southgate A; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Obiri-Yeboa I; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Jones LG; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Greco K; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Olayanju A; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Mbundi L; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Somasundaram M; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
  • Davidson B; Department of Surgery, Royal Free Campus, UCL Medical School, London, United Kingdom.
  • Sibbons PD; Tissue Engineering and Regenerative Medicine, Northwick Park Institute for Medical Research (NPIMR), Harrow, United Kingdom.
Stem Cells Dev ; 29(5): 314-326, 2020 03 01.
Article en En | MEDLINE | ID: mdl-31854227
The growing number of patients requiring liver transplantation for chronic liver disease cannot be currently met due to a shortage in donor tissue. As such, alternative tissue engineering approaches combining the use of acellular biological scaffolds and different cell populations (hepatic or progenitor) are being explored to augment the demand for functional organs. Our goal was to produce a clinically relevant sized scaffold from a sustainable source within 24 h, while preserving the extracellular matrix (ECM) to facilitate cell repopulation at a later stage. Whole porcine livers underwent perfusion decellularization via the hepatic artery and hepatic portal vein using a combination of saponin, sodium deoxycholate, and deionized water washes resulting in an acellular scaffold with an intact vasculature and preserved ECM. Molecular and immunohistochemical analysis (collagen I and IV and laminin) showed complete removal of any DNA material, together with excellent retention of glycosaminoglycans and collagen. Fourier-transform infrared spectroscopy (FTIR) analysis showed both absence of nuclear material and removal of any detergent residue, which was successfully achieved after additional ethanol gradient washes. Samples of the decellularized scaffold were assessed for cytotoxicity by seeding with porcine adipose-derived mesenchymal stem cells in vitro, these cells over a 10-day period showed attachment and proliferation. Perfusion of the vascular tree with contrast media followed by computed tomography (CT) imaging showed an intact vascular network. In vivo implantation of whole intact nonseeded livers, into a porcine model (as auxiliary graft) showed uniform perfusion macroscopically and histologically. Using this method, it is possible to create an acellular, clinically sized, liver scaffold with intact vasculature in less than 24 h.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido / Hígado Límite: Animals Idioma: En Revista: Stem Cells Dev Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido / Hígado Límite: Animals Idioma: En Revista: Stem Cells Dev Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido