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Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.
Henry, Matthew N; MacDonald, Michael A; Orellana, Camila A; Gray, Peter P; Gillard, Marianne; Baker, Kym; Nielsen, Lars K; Marcellin, Esteban; Mahler, Stephen; Martínez, Verónica S.
Afiliación
  • Henry MN; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • MacDonald MA; ARC Training Centre for Biopharmaceutical Innovation (CBI), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Orellana CA; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Gray PP; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Gillard M; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Baker K; ARC Training Centre for Biopharmaceutical Innovation (CBI), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Nielsen LK; Patheon Biologics-A Part of Thermo Fisher Scientific, Brisbane, Queensland, Australia.
  • Marcellin E; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Mahler S; ARC Training Centre for Biopharmaceutical Innovation (CBI), Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
  • Martínez VS; Metabolomics Australia, The University of Queensland, Brisbane, Queensland, Australia.
Biotechnol Bioeng ; 117(4): 1187-1203, 2020 04.
Article en En | MEDLINE | ID: mdl-31930480
ABSTRACT
Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Productos Biológicos / Células CHO / Apoptosis / Ingeniería Celular Límite: Animals Idioma: En Revista: Biotechnol Bioeng Año: 2020 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Productos Biológicos / Células CHO / Apoptosis / Ingeniería Celular Límite: Animals Idioma: En Revista: Biotechnol Bioeng Año: 2020 Tipo del documento: Article País de afiliación: Australia