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Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells.
Russo, Valentina; El Khatib, Mohammad; di Marcantonio, Lisa; Ancora, Massimo; Wyrwa, Ralf; Mauro, Annunziata; Walter, Torsten; Weisser, Jürgen; Citeroni, Maria Rita; Lazzaro, Francesco; Di Federico, Marta; Berardinelli, Paolo; Cammà, Cesare; Schnabelrauch, Matthias; Barboni, Barbara.
Afiliación
  • Russo V; Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
  • El Khatib M; Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
  • di Marcantonio L; Laboratory of Bacteriology, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "Giuseppe Caporale", 64100 Teramo, Italy.
  • Ancora M; Laboratory of Molecular Biology and Genomic, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "Giuseppe Caporale, 64100 Teramo, Italy.
  • Wyrwa R; Department of Biomaterials, INNOVENT e. V, J-07749 Jena, Germany.
  • Mauro A; Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
  • Walter T; Department of Biomaterials, INNOVENT e. V, J-07749 Jena, Germany.
  • Weisser J; Department of Biomaterials, INNOVENT e. V, J-07749 Jena, Germany.
  • Citeroni MR; Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
  • Lazzaro F; Research & Development Department, Assut Europe S.p.A., 67062 Magliano dei Marsi, L'Aquila, Italy.
  • Di Federico M; Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
  • Berardinelli P; Laboratory of Molecular Biology and Genomic, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "Giuseppe Caporale, 64100 Teramo, Italy.
  • Cammà C; Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.
  • Schnabelrauch M; Laboratory of Molecular Biology and Genomic, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "Giuseppe Caporale, 64100 Teramo, Italy.
  • Barboni B; Department of Biomaterials, INNOVENT e. V, J-07749 Jena, Germany.
Cells ; 9(2)2020 01 27.
Article en En | MEDLINE | ID: mdl-32012741
ABSTRACT
Background. The design of tendon biomimetic electrospun fleece with Amniotic Epithelial Stem Cells (AECs) that have shown a high tenogenic attitude may represent an alternative strategy to overcome the unsatisfactory results of conventional treatments in tendon regeneration. Methods. In this study, we evaluated AEC-engineered electrospun poly(lactide-co-glycolide) (PLGA) fleeces with highly aligned fibers (ha-PLGA) that mimic tendon extracellular matrix, their biocompatibility, and differentiation towards the tenogenic lineage. PLGA fleeces with randomly distributed fibers (rd-PLGA) were generated as control. Results. Optimal cell infiltration and biocompatibility with both PLGA fleeces were shown. However, only ha-PLGA fleeces committed AECs towards an Epithelial-Mesenchymal Transition (EMT) after 48 h culture, inducing their cellular elongation along the fibers' axis and the upregulation of mesenchymal markers. AECs further differentiated towards tenogenic lineage as confirmed by the up-regulation of tendon-related genes and Collagen Type 1 (COL1) protein expression that, after 28 days culture, appeared extracellularly distributed along the direction of ha-PLGA fibers. Moreover, long-term co-cultures of AEC-ha-PLGA bio-hybrids with fetal tendon explants significantly accelerated of half time AEC tenogenic differentiation compared to ha-PLGA fleeces cultured only with AECs. Conclusions. The fabricated tendon biomimetic ha-PLGA fleeces induce AEC tenogenesis through an early EMT, providing a potential tendon substitute for tendon engineering research.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre / Tendones / Diferenciación Celular / Ingeniería de Tejidos / Materiales Biomiméticos / Células Epiteliales / Transición Epitelial-Mesenquimal / Copolímero de Ácido Poliláctico-Ácido Poliglicólico / Amnios Límite: Animals Idioma: En Revista: Cells Año: 2020 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre / Tendones / Diferenciación Celular / Ingeniería de Tejidos / Materiales Biomiméticos / Células Epiteliales / Transición Epitelial-Mesenquimal / Copolímero de Ácido Poliláctico-Ácido Poliglicólico / Amnios Límite: Animals Idioma: En Revista: Cells Año: 2020 Tipo del documento: Article País de afiliación: Italia