Comparison of phenotypic methods with mecA gene based polymerase chain reaction for Methicillin-resistant Staphylococcus aureus detection.
J Pak Med Assoc
; 70(2): 276-280, 2020 Feb.
Article
en En
| MEDLINE
| ID: mdl-32063621
ABSTRACT
OBJECTIVE:
To determine methicillin resistance in staphylococcus aureus by different phenotypic methods, and to evaluate their accuracy with mecA gene polymerase chain reaction for methicillin-resistant staphylococcus aureus detection.METHODS:
The descriptive cross-sectional study was conducted from January to December 2015 at the Post- Graduate Medical Institute, Lahore, Pakistan, and comprised consecutive, non-repetitive clinical isolates of methicillin-resistant staphylococcus aureus that were screened with oxacillin disk 1µg and cefoxitin disk 30µg by Kirby-Bauer method using Clinical and Laboratory Standards Institute guideline. The isolates were cultured on oxacillin screen and mannitol salt agar, and subjected to latex agglutination for penicillin-binding protein 2aand polymerase chain reaction for mecA gene. Data was analysed using SPSS 20.RESULTS:
All the 105 isolates were resistant on oxacillin and cefoxitin disk diffusion test, but 95(90.47%) were positive for mecA gene by latex agglutination and polymerase chain reaction. The sensitivity of oxacillin salt agar, mannitol salt agar and latex agglutination was 94.31%, 96.73% and 98.95%, respectively. Keeping polymerase chain reaction as the gold standard, the specificity and diagnostic accuracy of latex agglutination were 77.77% and 97.14% respectively, which was the highest among all the phenotypic methods.CONCLUSIONS:
Latex agglutination method can be proposed as a swiftly reliable diagnostic technique for the detection of mecA gene in methicillin-resistant staphylococcus aureus isolates in resource-constrained settings where molecular methods are limited.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Infecciones Estafilocócicas
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Proteínas Bacterianas
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Pruebas de Fijación de Látex
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Reacción en Cadena de la Polimerasa
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Resistencia a la Meticilina
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Proteínas de Unión a las Penicilinas
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Pruebas Antimicrobianas de Difusión por Disco
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Staphylococcus aureus Resistente a Meticilina
Tipo de estudio:
Diagnostic_studies
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Guideline
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Observational_studies
Límite:
Humans
Idioma:
En
Revista:
J Pak Med Assoc
Año:
2020
Tipo del documento:
Article