Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine.
Proc Natl Acad Sci U S A
; 117(12): 6675-6685, 2020 03 24.
Article
en En
| MEDLINE
| ID: mdl-32152119
ABSTRACT
A comprehensive understanding of the development and evolution of human B cell responses induced by pathogen exposure will facilitate the design of next-generation vaccines. Here, we utilized a high-throughput single B cell cloning technology to longitudinally track the human B cell response to the yellow fever virus 17D (YFV-17D) vaccine. The early memory B cell (MBC) response was mediated by both classical immunoglobulin M (IgM) (IgM+CD27+) and switched immunoglobulin (swIg+) MBC populations; however, classical IgM MBCs waned rapidly, whereas swIg+ and atypical IgM+ and IgD+ MBCs were stable over time. Affinity maturation continued for 6 to 9 mo following vaccination, providing evidence for the persistence of germinal center activity long after the period of active viral replication in peripheral blood. Finally, a substantial fraction of the neutralizing antibody response was mediated by public clones that recognize a fusion loop-proximal antigenic site within domain II of the viral envelope glycoprotein. Overall, our findings provide a framework for understanding the dynamics and complexity of human B cell responses elicited by infection and vaccination.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fiebre Amarilla
/
Virus de la Fiebre Amarilla
/
Linfocitos B
/
Vacuna contra la Fiebre Amarilla
/
Memoria Inmunológica
/
Anticuerpos Antivirales
/
Antígenos Virales
Límite:
Adult
/
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2020
Tipo del documento:
Article