Vibiro vulnificus hemolysin associates with gangliosides.
BMC Microbiol
; 20(1): 69, 2020 03 30.
Article
en En
| MEDLINE
| ID: mdl-32228455
ABSTRACT
BACKGROUND:
Vibrio vulnificus hemolysin (VVH) is a pore-forming toxin secreted by Vibrio vulnificus. Cellular cholesterol was believed to be the receptor for VVH, because cholesterol could bind to VVH and preincubation with cholesterol inhibited cytotoxicity. It has been reported that specific glycans such as N-acetyl-D-galactosamine and N-acetyl-D-lactosamine bind to VVH, however, it has not been known whether these glycans could inhibit the cytotoxicity of VVH without oligomer formation. Thus, to date, binding mechanisms of VVH to cellular membrane, including specific receptors have not been elucidated.RESULTS:
We show here that VVH associates with ganglioside GM1a, Fucosyl-GM1, GD1a, GT1c, and GD1b by glycan array. Among them, GM1a could pulldown VVH. Moreover, the GD1a inhibited the cytotoxicity of VVH without the formation of oligomers.CONCLUSION:
This is the first report of a molecule able to inhibit the binding of VVH to target cells without oligomerization of VVH.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Membrana Celular
/
Vibrio vulnificus
/
Gangliósidos
/
Proteínas Hemolisinas
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
BMC Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón