Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells.
Nat Commun
; 11(1): 1609, 2020 03 30.
Article
en En
| MEDLINE
| ID: mdl-32231223
ABSTRACT
In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and -521 without the need for manual isolation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pigmentos Retinianos
/
Biomarcadores
/
Diferenciación Celular
/
Células Epiteliales
/
Neuronas
Tipo de estudio:
Diagnostic_studies
/
Guideline
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Suecia