Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum.
Biotechniques
; 69(1): 369-375, 2020 07.
Article
en En
| MEDLINE
| ID: mdl-32336113
ABSTRACT
Fusarium circinatum is the causal agent of pitch canker, a lethal disease of pine and other conifers. Since F. circinatum is a quarantine organism, its timely detection could efficiently prevent its introduction into new areas or facilitate spread management in already infected sites. In this study, we developed a sequence-specific probe loop-mediated isothermal amplification (LAMP) assay for F. circinatum using a field-deployable portable instrument. The assay was able to recognize the pathogen in host tissues in just 30 min, and the sensitivity of the assay made it possible to detect even small amounts of F. circinatum DNA (as low as 0.5 pg/µl). The high efficiency of this method suggests its use as a standard diagnostic tool during phytosanitary controls.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Amplificación de Ácido Nucleico
/
Técnicas de Diagnóstico Molecular
/
Fusarium
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biotechniques
Año:
2020
Tipo del documento:
Article
País de afiliación:
República Checa