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Cold-sensitive phenotypes of a yeast null mutant of ARV1 support its role as a GPI flippase.
Okai, Haruka; Ikema, Ryoko; Nakamura, Hiroki; Kato, Mei; Araki, Misako; Mizuno, Ayumi; Ikeda, Atsuko; Renbaum, Paul; Segel, Reeval; Funato, Kouichi.
Afiliación
  • Okai H; School of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
  • Ikema R; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
  • Nakamura H; Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
  • Kato M; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
  • Araki M; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
  • Mizuno A; School of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
  • Ikeda A; Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
  • Renbaum P; Medical Genetics Institute, Shaare Zedek Medical Center, Jerusalem, Israel.
  • Segel R; Medical Genetics Institute, Shaare Zedek Medical Center, Jerusalem, Israel.
  • Funato K; School of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
FEBS Lett ; 594(15): 2431-2439, 2020 08.
Article en En | MEDLINE | ID: mdl-32449190
Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediates from the cytoplasmic side into the luminal side of the ER membranes. Here, we show that the deletion of the ARV1 gene in yeast leads to cold-sensitive defects in cell growth and GPI anchor synthesis. Furthermore, complementation assays show that the overexpression of a missense human ARV1-G189R mutant does not completely restore the cold-sensitive phenotypes of the yeast arv1 mutant. Our results support the proposed role of Arv1 in GPI anchor synthesis and suggest that ARV1-linked human diseases result from defective GPI anchor synthesis.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Glicosilfosfatidilinositoles / Proteínas de Saccharomyces cerevisiae / Proteínas de la Membrana Tipo de estudio: Diagnostic_studies Idioma: En Revista: FEBS Lett Año: 2020 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Glicosilfosfatidilinositoles / Proteínas de Saccharomyces cerevisiae / Proteínas de la Membrana Tipo de estudio: Diagnostic_studies Idioma: En Revista: FEBS Lett Año: 2020 Tipo del documento: Article País de afiliación: Japón