Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane.
Angew Chem Int Ed Engl
; 59(37): 16122-16127, 2020 09 07.
Article
en En
| MEDLINE
| ID: mdl-32506656
ABSTRACT
Herein, we report the biosynthesis of protein heterocatenanes using a programmed sequence of multiple post-translational processing events including intramolecular chain entanglement, inâ
situ backbone cleavage, and spontaneous cyclization. The approach is general, autonomous, and can obviate the need for any additional enzymes. The catenane topology was convincingly proven using a combination of SDS-PAGE, LC-MS, size exclusion chromatography, controlled proteolytic digestion, and protein crystallography. The X-ray crystal structure clearly shows two mechanically interlocked protein rings with intact folded domains. It opens new avenues in the nascent field of protein-topology engineering.
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MEDLINE
Asunto principal:
Cristalografía por Rayos X
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Antracenos
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2020
Tipo del documento:
Article