Microfluidic particle zipper enables controlled loading of droplets with distinct particle types.
Lab Chip
; 20(14): 2465-2472, 2020 07 14.
Article
en En
| MEDLINE
| ID: mdl-32531004
ABSTRACT
Current encapsulation approaches control the number of particles encapsulated per droplet, but not the particle types; consequently, they are unable to generate droplets containing combinations of distinct particle types, limiting the reactions that can be performed. We describe a microfluidic particle zipper that allows the number and types of particles encapsulated in every droplet to be controlled. The approach exploits self-ordering to generate repeating particle patterns that allow controlled encapsulation in droplets. We use the method to combine barcode particles with gel encapsulated cells to profile multiple disease relevant genomic loci with single cell sequencing. Particle zippers can operate in series to generate complex particle compositions in droplets.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microfluídica
/
Técnicas Analíticas Microfluídicas
Idioma:
En
Revista:
Lab Chip
Asunto de la revista:
BIOTECNOLOGIA
/
QUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos