High-Aspect-Ratio SU-8-Based Optofluidic Device for Ammonia Detection in Cell Culture Media.
ACS Sens
; 5(8): 2523-2529, 2020 08 28.
Article
en En
| MEDLINE
| ID: mdl-32666799
ABSTRACT
Miniaturization of sensing technology has led to the development of multifunctional micro total analysis systems (µTAS) that benefit from microfluidic technology. Optical sensing is one of the most commonly used sensing approaches integrated into µTAS devices and features high sensitivity and low detection limits. Different materials have been used for the fabrication of µTAS devices, each having their advantages and disadvantages. Herein, a high-aspect-ratio optofluidic waveguide fabricated from SU-8 is presented for the first time. The suitable optical properties and chemical inertness of SU-8 provide a durable device made by a flexible and cost-efficient fabrication process. The optofluidic device was used for colorimetric ammonia (NH3) sensing with a dynamic range of 3-70 µM, a detection limit of 2.5 µM, a response time of 8 min, and close to 10 times better analytical performance compared to using a standard microplate reader. The µTAS device was capable of monitoring NH3 accumulating in the cell culture media of prostatic epithelial cell (BPH-1) culture.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Analíticas Microfluídicas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
ACS Sens
Año:
2020
Tipo del documento:
Article
País de afiliación:
Australia