Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids.
Cell Tissue Res
; 382(3): 463-476, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-32737576
ABSTRACT
Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organization and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, glutamatergic and serotonergic neurons. Recapitulating these in vivo features makes hMOs an excellent tool for in vitro disease phenotyping and drug discovery.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Organoides
/
Análisis de Secuencia de ARN
/
Neuronas Dopaminérgicas
/
Transcriptoma
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Cell Tissue Res
Año:
2020
Tipo del documento:
Article
País de afiliación:
Luxemburgo