CRISPR/Cas-based precision genome editing via microhomology-mediated end joining.
Plant Biotechnol J
; 19(2): 230-239, 2021 02.
Article
en En
| MEDLINE
| ID: mdl-33047464
ABSTRACT
Gene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide-directed mutagenesis (ODM), base editing, prime editing and especially homologous recombination (HR)-based gene targeting. With the advent of CRISPR/Cas for the targeted generation of DNA breaks (single-stranded breaks (SSBs) or double-stranded breaks (DSBs)), a substantial advancement in HR-mediated precise editing frequencies has been achieved. Nonetheless, further research needs to be performed for commercially viable applications of precise genome editing; hence, an alternative innovative method for genome editing may be required. Within this scope, we summarize recent progress regarding precision genome editing mediated by microhomology-mediated end joining (MMEJ) and discuss their potential applications in crop improvement.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
/
Edición Génica
Idioma:
En
Revista:
Plant Biotechnol J
Asunto de la revista:
BIOTECNOLOGIA
/
BOTANICA
Año:
2021
Tipo del documento:
Article