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The leukocyte non-coding RNA landscape in critically ill patients with sepsis.
Scicluna, Brendon P; Uhel, Fabrice; van Vught, Lonneke A; Wiewel, Maryse A; Hoogendijk, Arie J; Baessman, Ingelore; Franitza, Marek; Nürnberg, Peter; Horn, Janneke; Cremer, Olaf L; Bonten, Marc J; Schultz, Marcus J; van der Poll, Tom.
Afiliación
  • Scicluna BP; Amsterdam UMC, University of Amsterdam, Center for Experimental Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam, Netherlands.
  • Uhel F; Amsterdam UMC, University of Amsterdam, Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Amsterdam, Netherlands.
  • van Vught LA; Amsterdam UMC, University of Amsterdam, Center for Experimental Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam, Netherlands.
  • Wiewel MA; Amsterdam UMC, University of Amsterdam, Center for Experimental Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam, Netherlands.
  • Hoogendijk AJ; Amsterdam UMC, University of Amsterdam, Center for Experimental Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam, Netherlands.
  • Baessman I; Amsterdam UMC, University of Amsterdam, Center for Experimental Molecular Medicine, Amsterdam Infection & Immunity, Amsterdam, Netherlands.
  • Franitza M; Cologne Center for Genomics, University of Cologne, Cologne, Germany.
  • Nürnberg P; Cologne Center for Genomics, University of Cologne, Cologne, Germany.
  • Horn J; Cologne Center for Genomics, University of Cologne, Cologne, Germany.
  • Cremer OL; Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.
  • Bonten MJ; Amsterdam UMC, University of Amsterdam, Department of Intensive Care Medicine, Amsterdam, Netherlands.
  • Schultz MJ; Department of Intensive Care, University Medical Center Utrecht, Utrecht, Netherlands.
  • van der Poll T; Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, Netherlands.
Elife ; 92020 12 11.
Article en En | MEDLINE | ID: mdl-33305733
The extent of non-coding RNA alterations in patients with sepsis and their relationship to clinical characteristics, soluble mediators of the host response to infection, as well as an advocated in vivo model of acute systemic inflammation is unknown. Here we obtained whole blood from 156 patients with sepsis and 82 healthy subjects among whom eight were challenged with lipopolysaccharide in a clinically controlled setting (human endotoxemia). Via next-generation microarray analysis of leukocyte RNA we found that long non-coding RNA and, to a lesser extent, small non-coding RNA were significantly altered in sepsis relative to health. Long non-coding RNA expression, but not small non-coding RNA, was largely recapitulated in human endotoxemia. Integrating RNA profiles and plasma protein levels revealed known as well as previously unobserved pathways, including non-sensory olfactory receptor activity. We provide a benchmark dissection of the blood leukocyte 'regulome' that can facilitate prioritization of future functional studies.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedad Crítica / Sepsis / ARN no Traducido / Leucocitos Tipo de estudio: Observational_studies / Prognostic_studies Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Elife Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedad Crítica / Sepsis / ARN no Traducido / Leucocitos Tipo de estudio: Observational_studies / Prognostic_studies Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Elife Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos